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Precise label-free leukocyte subpopulations separation using hybrid acoustic-optical chip


Leukocytes subpopulations contain crucial physiology information, hence precise specific leukocyte separation is very important for leukemia diagnosis and analysis. However, the conventional centrifugation and immunofluorescence-based separation methods are inaccurate and inconvenient due to the overlapping cell size and density or complex marking process. Here we report a new label-free technology for precise leukocyte subpopulations separation by synergy of acoustic and optical technologies. Surface acoustic wave (SSAW) solved the problem of gente and precise focusing cells in optical system, besides, SSAW is also used for the separation of granulocytes which have obviouse size distinction from other components. While for lymphocyte and monocyte which have overlap in size/density, optical force could distinguish them accurately based on RI difference with the covenience of acoustic pre-focusing part. The experiment conducts separation of three kinds of leukocyte subtypes with considerable throughput and purity, we obtain 99% pure lymphocytes, 98% pure monocytes, and 95% pure granulocytes. Experimental results prove the device has robust ability in leukocytes phenotypes separation, with the advantage of non-invasive, label-free and precise. In the future, this convenient hybrid method will also be a potential powerful tool for clinical auxiliary diagnosis and analysis.

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Publication details

The article was received on 28 Aug 2018, accepted on 12 Oct 2018 and first published on 12 Oct 2018

Article type: Paper
DOI: 10.1039/C8LC00911B
Citation: Lab Chip, 2018, Accepted Manuscript
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    Precise label-free leukocyte subpopulations separation using hybrid acoustic-optical chip

    X. Hu, H. Liu, Y. Jin, L. Liang, D. Zhu, X. Zhu, S. Guo, F. Zhou and Y. Yang, Lab Chip, 2018, Accepted Manuscript , DOI: 10.1039/C8LC00911B

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