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Self-Filling Microwell Arrays (SFMAs) for Tumor Spheroid Formation

Abstract

Tumor spheroids formation in microwell arrays is a promising approach for high-throughput screening of chemotherapeutic agents. This method offers the advantage of better mimicking the complexities of tumors as compared to conventional monolayer culture systems. However, using these technologies to their full potential is hindered by the inability to seed the cells within the wells uniformly and with high yield and reproducibility. Moreover, standard manufacturing approaches for fabrication of microwell arrays rely on lithography and etching techniques, which are costly, labor-intensive, and time-consuming. Herein, we report on the development of self-filling microwell arrays (SFMWs) in which cells are directed from a loading chamber to microwells using inclined guiding channels. SFMWs are fabricated by replica molding of a three-dimensionally (3D) printed molds in agarose. We characterize the fabrication process and demonstrate the ability to culture breast adenocarcinoma MCF-7 and U87 glioma in SFMWs and perform drug toxicity studies. We envision that the proposed innovative approach opens avenues of opportunities for high-throughput three-dimensional cell culture for drug screening and disease modeling.

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Supplementary files

Publication details

The article was received on 06 Jul 2018, accepted on 06 Oct 2018 and first published on 08 Oct 2018


Article type: Paper
DOI: 10.1039/C8LC00708J
Citation: Lab Chip, 2018, Accepted Manuscript
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    Self-Filling Microwell Arrays (SFMAs) for Tumor Spheroid Formation

    A. Seyfoori, E. Samiei, B. Godau, N. Jalili, M. rahmanian, L. Farahmand, K. Majidzadeh and M. Akbari, Lab Chip, 2018, Accepted Manuscript , DOI: 10.1039/C8LC00708J

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