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Microfluidic bead encapsulation above 20 kHz with triggered drop formation

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Abstract

Microsphere beads are functionalized with oligonucleotides, antibodies, and other moieties to enable specific detection of analytes. Droplet microfluidics leverages this for single-molecule or -cell analysis by pairing beads and targets in water-in-oil droplets. Pairing is achieved with devices operating in the dripping regime, limiting throughput. Here, we describe a pairing method that uses beads to trigger the breakup of a jet into monodispersed droplets. We use the method to pair 105 Human T cells with polyacrylamide beads ten times faster than methods operating in the dripping regime. Our method improves the throughput of bead-based droplet workflows, enabling analysis of large populations and the detection of rare events.

Graphical abstract: Microfluidic bead encapsulation above 20 kHz with triggered drop formation

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Publication details

The article was received on 21 May 2018, accepted on 03 Oct 2018 and first published on 26 Oct 2018


Article type: Paper
DOI: 10.1039/C8LC00514A
Citation: Lab Chip, 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    Microfluidic bead encapsulation above 20 kHz with triggered drop formation

    I. C. Clark and A. R. Abate, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00514A

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