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Issue 20, 2018
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3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade

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Abstract

Microfluidic culture has the potential to revolutionize cancer diagnosis and therapy. Indeed, several microdevices are being developed specifically for clinical use to test novel cancer therapeutics. To be effective, these platforms need to replicate the continuous interactions that exist between tumor cells and non-tumor cell elements of the tumor microenvironment through direct cell–cell or cell–matrix contact or by the secretion of signaling factors such as cytokines, chemokines and growth factors. Given the challenges of personalized or precision cancer therapy, especially with the advent of novel immunotherapies, a critical need exists for more sophisticated ex vivo diagnostic systems that recapitulate patient-specific tumor biology with the potential to predict response to immune-based therapies in real-time. Here, we present details of a method to screen for the response of patient tumors to immune checkpoint blockade therapy, first reported in Jenkins et al. Cancer Discovery, 2018, 8, 196–215, with updated evaluation of murine- and patient-derived organotypic tumor spheroids (MDOTS/PDOTS), including evaluation of the requirement for 3D microfluidic culture in MDOTS, demonstration of immune-checkpoint sensitivity of PDOTS, and expanded evaluation of tumor–immune interactions using RNA-sequencing to infer changes in the tumor–immune microenvironment. We also examine some potential improvements to current systems and discuss the challenges in translating such diagnostic assays to the clinic.

Graphical abstract: 3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade

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Publication details

The article was received on 29 Mar 2018, accepted on 22 Aug 2018 and first published on 05 Sep 2018


Article type: Paper
DOI: 10.1039/C8LC00322J
Citation: Lab Chip, 2018,18, 3129-3143
  • Open access: Creative Commons BY-NC license
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    3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade

    A. R. Aref, M. Campisi, E. Ivanova, A. Portell, D. Larios, B. P. Piel, N. Mathur, C. Zhou, R. V. Coakley, A. Bartels, M. Bowden, Z. Herbert, S. Hill, S. Gilhooley, J. Carter, I. Cañadas, T. C. Thai, S. Kitajima, V. Chiono, C. P. Paweletz, D. A. Barbie, R. D. Kamm and R. W. Jenkins, Lab Chip, 2018, 18, 3129
    DOI: 10.1039/C8LC00322J

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