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Issue 11, 2018
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Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions

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Abstract

Neutrophils are the first white blood cells to respond to microbes and to limit their invasion of the body. However, the growth of the microbes into colonies often challenges the neutrophils ability to contain them. To study the interactions between neutrophils and microbial colonies, we designed an assay for arranging microbes in clusters of controlled size (i.e. living colloids). The patterned microbes in the living colloid are mechanically trapped inside the wells and fully accessible to neutrophils. Using the assay, we studied the interactions between human neutrophils and Candida albicans and Staphylococcus aureus, two common human pathogens. We also probed the susceptibility of C. albicans colloids to caspofungin, a common antifungal drug.

Graphical abstract: Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions

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Publication details

The article was received on 05 Mar 2018, accepted on 08 May 2018 and first published on 16 May 2018


Article type: Communication
DOI: 10.1039/C8LC00228B
Citation: Lab Chip, 2018,18, 1514-1520
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    Large-scale patterning of living colloids for dynamic studies of neutrophil–microbe interactions

    J. J. Kim, E. Reátegui, A. Hopke, F. Jalali, M. Roushan, P. S. Doyle and D. Irimia, Lab Chip, 2018, 18, 1514
    DOI: 10.1039/C8LC00228B

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