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Issue 10, 2018
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A microfluidic biochip platform for electrical quantification of proteins

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Abstract

Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. Several immune biomarker levels, including Interleukin 6 (IL-6), have shown a high correlation to the onset and progression of sepsis. Currently, no technology diagnoses and stratifies sepsis progression using biomarker levels. This paper reports a microfluidic biochip platform to detect proteins in undiluted human plasma samples. The device uses a differential enumeration platform that integrates Coulter counting principles, antigen specific capture chambers, and micro size bead based immunodetection to quantify cytokines. This microfluidic biochip was validated as a potential point of care technology by quantifying IL-6 from plasma samples (n = 29) with good correlation (R2 = 0.81) and agreement (Bland–Altman) compared to controls. In combination with previous applications, this point of care platform can potentially detect cell and protein biomarkers simultaneously for sepsis stratification.

Graphical abstract: A microfluidic biochip platform for electrical quantification of proteins

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Supplementary files

Article information


Submitted
12 Jan 2018
Accepted
06 Apr 2018
First published
10 Apr 2018

Lab Chip, 2018,18, 1461-1470
Article type
Paper

A microfluidic biochip platform for electrical quantification of proteins

E. Valera, J. Berger, U. Hassan, T. Ghonge, J. Liu, M. Rappleye, J. Winter, D. Abboud, Z. Haidry, R. Healey, N. Hung, N. Leung, N. Mansury, A. Hasnain, C. Lannon, Z. Price, K. White and R. Bashir, Lab Chip, 2018, 18, 1461
DOI: 10.1039/C8LC00033F

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