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Issue 4, 2018
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Fluorescence in sub-10 nm channels with an optical enhancement layer

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Abstract

Fluorescence microscopy uniquely enables physical and biological research in micro- and nanofluidic systems. However, in channels with depths below 10 nm, the limited number of fluorophores results in fluorescence intensity below the detection limit of optical microscopes. To overcome this barrier, we applied Fabry–Pérot interference to enhance fluorescence intensity with a silicon nitride layer below the sub-10 nm channel. A silicon nitride layer of suitable thickness can selectively enhance both absorption and emission wavelengths, leading to a fluorescent signal that is enhanced 20-fold and readily imaged with traditional microscopes. To demonstrate this method, we studied the mass transport of a binary solution of ethanol and Rhodamin B in 8 nm nanochannels. The large molecular size of Rhodamin B (∼1.8 nm) relative to the channel depth results in both separation and reduced diffusivity, deviating from behavior at larger scales. This method extends the widely available suite of fluorescence analysis tools and infrastructure to unprecedented sub-10 nm scale with relevance to a wide variety of biomolecular interactions.

Graphical abstract: Fluorescence in sub-10 nm channels with an optical enhancement layer

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Publication details

The article was received on 09 Nov 2017, accepted on 21 Jan 2018 and first published on 22 Jan 2018


Article type: Communication
DOI: 10.1039/C7LC01193H
Citation: Lab Chip, 2018,18, 568-573
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    Fluorescence in sub-10 nm channels with an optical enhancement layer

    J. Zhong, S. Talebi, Y. Xu, Y. Pang, F. Mostowfi and D. Sinton, Lab Chip, 2018, 18, 568
    DOI: 10.1039/C7LC01193H

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