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The Protective Effects of Selenium-Enriched Spirulina on the Reproductive System of Male Zebrafish (Danio rerio) Exposed to Beta-Cypermethrin

Abstract

Beta-cypermethrin (beta-CYP), which is widely used for the control of indoor and field pests, is a highly lipophilic insecticide with environmental estrogenic effects. Selenium-enriched Spirulina (Se-SP) is a novel natural antioxidant agent with high nutritive value. This study aimed to investigate the protective effects of Se-SP on the reproductive system of male zebrafish (Danio rerio) exposed to beta-CYP. We determined the protective effect of Se-SP on male zebrafish exposed to 7 nmol/L beta-CYP by measuring the activity of sperm and antioxidants, examining tissue sections, measuring hormone secretion levels, and analyzing the expression of related proteins and genes during spermatogenesis. We found that beta-CYP enhanced VTG synthesis and estrogen (E2) secretion, increased the number of spermatogonia and spermatocytes, and decreased the number of spermatids and Sertoli cells. However, treatment of 50 mg/L Se-SP (Se:25 μmol/g Se-SP) almost completely ameliorated the deleterious effects of beta-CYP. The protective mechanism of Se-SP in the testes of beta-CYP-treated fish involved enhanced of antioxidant enzyme activity and increased androgen secretion by downregulation of the expression of cyp19a, nanos2, piwil1, dazl and sycp3, and upregulation of the expression of odf3b, igf3 and dmrt1, which affected the testosterone production and spermatogenesis. Our study strongly indicates that treatment with Se-SP can effectively prevent the oxidative damage to the reproductive system during spermatocytes differentiation induced by beta-CYP exposure.

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Publication details

The article was received on 29 Jul 2018, accepted on 06 Oct 2018 and first published on 10 Oct 2018


Article type: Paper
DOI: 10.1039/C8FO01527A
Citation: Food Funct., 2018, Accepted Manuscript
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    The Protective Effects of Selenium-Enriched Spirulina on the Reproductive System of Male Zebrafish (Danio rerio) Exposed to Beta-Cypermethrin

    Y. Zhang, Y. Zhou, Q. Tang, F. Hu, L. Feng, J. Shen and B. Huang, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO01527A

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