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CuO and ZnO nanoparticles drive the propagation of antibiotic resistance genes during sludge anaerobic digestion: possible role of stimulated signal transduction

Abstract

Emerging contaminants, such as engineered metal oxides nanoparticles (NPs) and antibiotic resistance genes (ARGs), were reported to be accumulated in waste activated sludge. In this study, the potential impacts of CuO and ZnO NPs on the fate of ARGs during sludge anaerobic digestion were assessed. Metagenomic results showed that the presence of CuO or ZnO NPs increased ARGs abundances but had no significant effects on the percentages of resistance mechanisms and types. Further functional analysis of the metagenomes revealed that signal transduction were stimulated by both NPs, especially two-component regulatory systems (TCRS) responsible for quorum sensing (QS), pili synthesis and metal tolerance. Moreover, it was found that the activated QS was closely associated with the shifted bacterial communities, especially the enriched genera, such as Acidovorax, Burkholderia, Pseudomonas and Rhodobacter, which were not only the producers of QS signals but also the hosts of ARGs. Meanwhile, the triggered pili synthesis, combining with the increased abundances of mobile genetic elements (plasmids, integrons and insertion sequences), would facilitate the transfer of ARGs among diverse bacteria. Furthermore, the induced resistance to copper/zinc and other metal, such as mercury and arsenic, probably promoted the co-selection between metal and antibiotic resistance. The above results indicated that the propagation of ARGs in digesters might be driven by the NPs-stimulated signal transduction. These findings may bring new insights to the understanding of ARGs responses to diverse environmental stimuli.

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Publication details

The article was received on 01 Apr 2018, accepted on 05 Dec 2018 and first published on 06 Dec 2018


Article type: Paper
DOI: 10.1039/C8EN00370J
Citation: Environ. Sci.: Nano, 2018, Accepted Manuscript
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    CuO and ZnO nanoparticles drive the propagation of antibiotic resistance genes during sludge anaerobic digestion: possible role of stimulated signal transduction

    H. Huang, Y. Chen, S. Yang and X. Zheng, Environ. Sci.: Nano, 2018, Accepted Manuscript , DOI: 10.1039/C8EN00370J

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