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Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

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Abstract

[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

Graphical abstract: Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

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Publication details

The article was received on 06 Jul 2018, accepted on 21 Sep 2018 and first published on 25 Sep 2018


Article type: Communication
DOI: 10.1039/C8EE01975D
Citation: Energy Environ. Sci., 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

    A. Wegelius, N. Khanna, C. Esmieu, G. D. Barone, F. Pinto, P. Tamagnini, G. Berggren and P. Lindblad, Energy Environ. Sci., 2018, Advance Article , DOI: 10.1039/C8EE01975D

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