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Issue 14, 2018
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Engineering enzyme microenvironments for enhanced biocatalysis

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Abstract

Protein engineering provides a means to alter protein structure leading to new functions. Much work has focused on the engineering of enzyme active sites to enhance catalytic activity, however there is an increasing trend towards engineering other aspects of biocatalysts as these efforts can also lead to useful improvements. This tutorial discusses recent advances in engineering an enzyme's local chemical and physical environment, with the goal of enhancing enzyme reaction kinetics, substrate selectivity, and activity in harsh conditions (e.g., low or high pH). By introducing stimuli-responsiveness to these enzyme modifications, dynamic control of activity also becomes possible. These new biomolecular and protein engineering techniques are separate and independent from traditional active site engineering and can therefore be applied synergistically to create new biocatalyst technologies with novel functions.

Graphical abstract: Engineering enzyme microenvironments for enhanced biocatalysis

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Publication details

The article was received on 29 Jan 2018 and first published on 25 May 2018


Article type: Tutorial Review
DOI: 10.1039/C8CS00085A
Citation: Chem. Soc. Rev., 2018,47, 5177-5186
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    Engineering enzyme microenvironments for enhanced biocatalysis

    L. Lancaster, W. Abdallah, S. Banta and I. Wheeldon, Chem. Soc. Rev., 2018, 47, 5177
    DOI: 10.1039/C8CS00085A

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