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Issue 9, 2018

Rapid localized crystallization of lysozyme by laser trapping

Author affiliations

Abstract

Confining protein crystallization to a millimetre size was achieved within 0.5 h after stopping 1 h intense trapping laser irradiation, which shows excellent performance in spatial and temporal controllability compared to spontaneous nucleation. A continuous-wave near-infrared laser beam is tightly focused into a glass/solution interfacial layer of a supersaturated buffer solution of hen egg-white lysozyme (HEWL). The crystallization is not observed during laser trapping, but initiated by stopping the laser irradiation. The generated crystals are localized densely in a circular area with a diameter of a few millimetres around the focal spot and show specific directions of the optical axes of the HEWL crystals. To interpret this unique crystallization, we propose a mechanism that nucleation and the subsequent growth take place in a highly concentrated domain consisting of HEWL liquid-like clusters after turning off laser trapping.

Graphical abstract: Rapid localized crystallization of lysozyme by laser trapping

Supplementary files

Article information


Submitted
13 Oct 2017
Accepted
03 Jan 2018
First published
03 Jan 2018

Phys. Chem. Chem. Phys., 2018,20, 6034-6039
Article type
Communication

Rapid localized crystallization of lysozyme by laser trapping

K. Yuyama, K. Chang, J. Tu, H. Masuhara and T. Sugiyama, Phys. Chem. Chem. Phys., 2018, 20, 6034 DOI: 10.1039/C7CP06990A

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