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Issue 98, 2018
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Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

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Abstract

A novel, simple, one-step and one-tube detection method was developed for ultrasensitive detection of polynucleotide kinase (PNK) activity on the basis of dual enzyme-synergistic signal amplification. This method was also demonstrated to work well for PNK inhibitor screening and endogenous PNK detection in cell lysates at a single-cell level.

Graphical abstract: Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

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Publication details

The article was received on 28 Oct 2018, accepted on 16 Nov 2018 and first published on 16 Nov 2018


Article type: Communication
DOI: 10.1039/C8CC08616H
Citation: Chem. Commun., 2018,54, 13841-13844
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    Dual enzyme-assisted one-step isothermal real-time amplification assay for ultrasensitive detection of polynucleotide kinase activity

    X. Li, Y. Du, Y. Pan, L. Su, S. Shi, S. Wang, A. Tang, K. Kim and D. Kong, Chem. Commun., 2018, 54, 13841
    DOI: 10.1039/C8CC08616H

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