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Issue 98, 2018
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A triarylphosphine–trimethylpiperidine reagent for the one-step derivatization and enrichment of protein post-translational modifications and identification by mass spectrometry

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Abstract

We report a new reagent that is capable of both chemical derivatization and selective enrichment of azide-labeled PTM peptides for sensitive identification by mass spectrometry (MS). Facile sample recovery, enhanced ionization and fragmentation in MS of the enriched PTM peptides are achieved, which leads to the identification of 3293 O-GlcNAc peptides and the location of 1706 sites in HeLa cells and efficiently expands the current mapping scale.

Graphical abstract: A triarylphosphine–trimethylpiperidine reagent for the one-step derivatization and enrichment of protein post-translational modifications and identification by mass spectrometry

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Publication details

The article was received on 21 Oct 2018, accepted on 23 Oct 2018 and first published on 02 Nov 2018


Article type: Communication
DOI: 10.1039/C8CC08416E
Citation: Chem. Commun., 2018,54, 13790-13793
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    A triarylphosphine–trimethylpiperidine reagent for the one-step derivatization and enrichment of protein post-translational modifications and identification by mass spectrometry

    B. Huo, W. Zhang, X. Zhao, H. Dong, Y. Yu, J. Wang, X. Qian and W. Qin, Chem. Commun., 2018, 54, 13790
    DOI: 10.1039/C8CC08416E

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