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A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

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Abstract

Metal complexes can initiate DNA cleavage by the formation of reactive oxygen species (ROS). A conventional assay to probe for ROS is to add quenchers in a gel electrophoresis experiment. As we show here, such an assay is neither selective nor reliable. Instead, we suggest the use of simple fluorogens, as tested here with several metallonucleases for the detection of H2O2 and HO˙.

Graphical abstract: A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

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Publication details

The article was received on 29 Aug 2018, accepted on 06 Nov 2018 and first published on 06 Nov 2018


Article type: Communication
DOI: 10.1039/C8CC06996D
Citation: Chem. Commun., 2018, Advance Article
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    A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

    S. Leichnitz, J. Heinrich and N. Kulak, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC06996D

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