A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

Abstract

Metal complexes can initiate DNA cleavage by the formation of reactive oxygen species (ROS). A conventional assay to probe for ROS is to add quenchers in a gel electrophoresis experiment. As we show here, such an assay is neither selective nor reliable. Instead, we suggest the use of simple fluorogens, as tested here with several metallonucleases for the detection of H₂O₂ and HO˙.