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Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

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Abstract

We report the functional synthesis and quantification of membrane proteins—α-hemolysin from Staphylococcus aureus and the multidrug transporter EmrE from Escherichia coli—at the stabilized droplet interface bilayer using an in vitro transcription–translation system. The system developed here can expand the list of integral membrane proteins applicable for quantitative functional analysis.

Graphical abstract: Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

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Publication details

The article was received on 22 Aug 2018, accepted on 05 Oct 2018 and first published on 08 Oct 2018


Article type: Communication
DOI: 10.1039/C8CC06804F
Citation: Chem. Commun., 2018, Advance Article
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    Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

    M. A. Elfaramawy, S. Fujii, A. Uyeda, T. Osaki, S. Takeuchi, Y. Kato, H. Watanabe and T. Matsuura, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC06804F

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