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Issue 97, 2018
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Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs

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Abstract

Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.

Graphical abstract: Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs

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Publication details

The article was received on 02 Aug 2018, accepted on 06 Sep 2018 and first published on 19 Nov 2018


Article type: Communication
DOI: 10.1039/C8CC06284F
Citation: Chem. Commun., 2018,54, 13702-13705
  • Open access: Creative Commons BY license
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    Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs

    N. Hellwig, O. Peetz, Z. Ahdash, I. Tascón, P. J. Booth, V. Mikusevic, M. Diskowski, A. Politis, Y. Hellmich, I. Hänelt, E. Reading and N. Morgner, Chem. Commun., 2018, 54, 13702
    DOI: 10.1039/C8CC06284F

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