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Issue 90, 2018
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Efficient cosubstrate enzyme pairs for sequence-specific methyltransferase-directed photolabile caging of DNA

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Abstract

Supplemented with synthetic surrogates of their natural cosubstrate S-adenosyl-L-methione (AdoMet), methyltransferases represent a powerful toolbox for the functionalization of biomolecules. By employing novel cosubstrate derivatives in combination with protein engineering, we show that this chemo-enzymatic method can be used to introduce photolabile protecting groups into DNA even in the presence of AdoMet. This approach enables optochemical control of gene expression in a straight-forward manner and we have termed it reversible methyltransferase directed transfer of photoactivatable groups (re-mTAG).

Graphical abstract: Efficient cosubstrate enzyme pairs for sequence-specific methyltransferase-directed photolabile caging of DNA

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Publication details

The article was received on 20 Jul 2018, accepted on 18 Oct 2018 and first published on 19 Oct 2018


Article type: Communication
DOI: 10.1039/C8CC05913F
Citation: Chem. Commun., 2018,54, 12718-12721
  • Open access: Creative Commons BY license
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    Efficient cosubstrate enzyme pairs for sequence-specific methyltransferase-directed photolabile caging of DNA

    M. Heimes, L. Kolmar and C. Brieke, Chem. Commun., 2018, 54, 12718
    DOI: 10.1039/C8CC05913F

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