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Issue 97, 2018
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Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins

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Abstract

Ceramide plays key roles in autophagy, inflammation and apoptosis. However, little is known about the molecular mechanisms regulating its function and only a handful of cellular effectors are known for this lipid. Here we show that azide-tagged sphingolipids are powerful tools to identify ceramide targets. The combination of a protein array analysis and a mass spectrometry-based proteomic profiling successfully detects known ceramide-binding proteins and identifies others not yet reported, several of which we validated using a variety of techniques.

Graphical abstract: Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins

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Publication details

The article was received on 12 Sep 2018, accepted on 12 Nov 2018 and first published on 20 Nov 2018


Article type: Communication
DOI: 10.1039/C8CC05691A
Chem. Commun., 2018,54, 13742-13745

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    Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins

    J. Schulte-Zweckel, T. Schneidewind, J. L. Abad, A. Brockmeyer, P. Janning and G. Triola, Chem. Commun., 2018, 54, 13742
    DOI: 10.1039/C8CC05691A

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