A triple-amplification strategy for sensitive detection of telomerase at the single-cell level

Abstract

We develop a triple-amplification strategy for sensitive detection of telomerase from cancer cells using telomere-based primer generation-triggered rolling circle amplification in combination with enzyme-assisted cyclic signal amplification. This method can detect telomerase at the single-cell level, and it can be further applied for the screening of telomerase inhibitors for anticancer drug discovery.