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Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

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Abstract

The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

Graphical abstract: Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

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Publication details

The article was received on 21 Jun 2018, accepted on 02 Oct 2018 and first published on 05 Oct 2018


Article type: Communication
DOI: 10.1039/C8CC04960B
Citation: Chem. Commun., 2018, Advance Article
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    Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

    W. Y. Chow, R. Li, I. Goldberga, D. G. Reid, R. Rajan, J. Clark, H. Oschkinat, M. J. Duer, R. Hayward and C. M. Shanahan, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC04960B

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