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Issue 61, 2018
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‘AND’-based fluorescence scaffold for the detection of ROS/RNS and a second analyte

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Abstract

Traditionally, fluorescence probes have focused on the detection of a single biomarker for a specific process. In this work, we set out to develop a number of fluorescence probes that enable the detection of a chosen analyte in the presence of reactive oxygen/nitrogen species (ROS/RNS). These fluorescence probes when activated result in the formation of the highly fluorescent pink dye, resorufin. Therefore, we have labelled these fluorescent probes as ‘Pinkments’. Our first ‘Pinkment’ was shown to detect biologically relevant concentrations of ONOO and have an excellent selectivity against other ROS/RNS. Pinkment-OH was developed to provide a core unit which could be easily functionalised to produce a range of ‘AND’ based fluorescence probes for the detection of ROS/RNS and a second analyte. For proof of concept, we synthesised Pinkment-OTBS and Pinkment-OAc. These ‘AND’-based probes were successfully shown to detect ROS/RNS and F or esterase, respectively.

Graphical abstract: ‘AND’-based fluorescence scaffold for the detection of ROS/RNS and a second analyte

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Publication details

The article was received on 30 May 2018, accepted on 26 Jun 2018 and first published on 27 Jun 2018


Article type: Communication
DOI: 10.1039/C8CC04316G
Citation: Chem. Commun., 2018,54, 8466-8469
  • Open access: Creative Commons BY license
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    ‘AND’-based fluorescence scaffold for the detection of ROS/RNS and a second analyte

    M. L. Odyniec, A. C. Sedgwick, A. H. Swan, M. Weber, T. M. S. Tang, J. E. Gardiner, M. Zhang, Y. Jiang, G. Kociok-Kohn, R. B. P. Elmes, S. D. Bull, X. He and T. D. James, Chem. Commun., 2018, 54, 8466
    DOI: 10.1039/C8CC04316G

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