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Issue 50, 2018
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Application of lanthanide luminescence in probing enzyme activity

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Enzymes play critical roles in the regulation of cellular function and are implicated in numerous disease conditions. Reliable and practicable assays are required to study enzyme activity, to facilitate the discovery of inhibitors and activators of enzymes related to disease. In recent years, a variety of enzyme assays have been devised that utilise luminescent lanthanide(III) complexes, taking advantage of their high detection sensitivities, long luminescence lifetimes, and line-like emission spectra that permit ratiometric and time-resolved analyses. In this Feature article, we focus on recent progress in the development of enzyme activity assays based on lanthanide(III) luminescence, covering a variety of strategies including Ln(III)-labelled antibodies and proteins, Ln(III) ion encapsulation within defined peptide sequences, reactivity-based Ln(III) probes, and discrete Ln(III) complexes. Emerging approaches for monitoring enzyme activity are discussed, including the use of anion responsive lanthanide(III) complexes, capable of molecular recognition and luminescence signalling of polyphosphate anions.

Graphical abstract: Application of lanthanide luminescence in probing enzyme activity

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Publication details

The article was received on 09 Apr 2018, accepted on 10 May 2018 and first published on 15 May 2018

Article type: Feature Article
DOI: 10.1039/C8CC02824A
Citation: Chem. Commun., 2018,54, 6635-6647
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    Application of lanthanide luminescence in probing enzyme activity

    S. H. Hewitt and S. J. Butler, Chem. Commun., 2018, 54, 6635
    DOI: 10.1039/C8CC02824A

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