XNA ligation using T4 DNA ligase in crowding conditions

Donaat Kestemont; Marleen Renders; Piotr Leonczak; Mikhail Abramov; Guy Schepers; Vitor B. Pinheiro; Jef Rozenski; Piet Herdewijn

doi:10.1039/C8CC02414F

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XNA ligation using T4 DNA ligase in crowding conditions†

Donaat Kestemont,

‡^a Marleen Renders,

‡*^a Piotr Leonczak,

^a Mikhail Abramov,

^a Guy Schepers,^a Vitor B. Pinheiro,

^bc Jef Rozenski^a and Piet Herdewijn

^ad

Author affiliations

* Corresponding authors

^a Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, Herestraat 49 box 1041, 3000 Leuven, Belgium
E-mail: marleen.renders@kuleuven.be
Fax: +32 16330026
Tel: +32 16322666

^b Structural and Molecular Biology Department, University College London, Gower Street, London, UK

^c Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, University of London, Malet Street, London, UK

^d Université d’Evry, CNRS-UMR8030/Laboratoire iSSB, CEA, DRF, IG, Genoscope, Université Paris-Saclay, Evry 91000, France

Abstract

T4 DNA ligase is capable of ligating 2′OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.

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Supplementary files

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Article information

DOI: https://doi.org/10.1039/C8CC02414F
Article type: Communication
Submitted: 26 Mar 2018
Accepted: 30 May 2018
First published: 31 May 2018

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Chem. Commun., 2018,54, 6408-6411

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XNA ligation using T4 DNA ligase in crowding conditions

D. Kestemont, M. Renders, P. Leonczak, M. Abramov, G. Schepers, V. B. Pinheiro, J. Rozenski and P. Herdewijn, Chem. Commun., 2018, 54, 6408 DOI: 10.1039/C8CC02414F

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Chemical Communications

XNA ligation using T4 DNA ligase in crowding conditions†

Abstract

Supplementary files

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XNA ligation using T4 DNA ligase in crowding conditions

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