Real-time monitoring of the aggregation of Alzheimer's amyloid-β via1H magic angle spinning NMR spectroscopy

Jian Wang; Tomoya Yamamoto; Jia Bai; Sarah J. Cox; Kyle J. Korshavn; Martine Monette; Ayyalusamy Ramamoorthy

doi:10.1039/C8CC00167G

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Real-time monitoring of the aggregation of Alzheimer's amyloid-β via¹H magic angle spinning NMR spectroscopy†

Jian Wang,‡^ab Tomoya Yamamoto,‡^ab Jia Bai,^ab Sarah J. Cox,^b Kyle J. Korshavn,^ab Martine Monette^c and Ayyalusamy Ramamoorthy

*^ab

Author affiliations

* Corresponding authors

^a Biophysics Program, The University of Michigan, Ann Arbor, MI 48109-1055, USA

^b Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA
E-mail: ramamoor@umich.edu

^c Bruker Canada Ltd, 2800 High Point Drive, Milton, Ontario, Canada

Abstract

Proton magic-angle-spinning NMR used for real-time analysis of amyloid aggregation reveals that mechanical rotation of Aβ_1–40 monomers increases the rate of formation of aggregates, and that the increasing lag-time with peptide concentration suggests the formation of growth-incompetent species. EGCG's ability to shift off-pathway aggregation is also demonstrated.

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Article information

DOI: https://doi.org/10.1039/C8CC00167G
Article type: Communication
Submitted: 08 Jan 2018
Accepted: 31 Jan 2018
First published: 31 Jan 2018

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Chem. Commun., 2018,54, 2000-2003

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Real-time monitoring of the aggregation of Alzheimer's amyloid-β via¹H magic angle spinning NMR spectroscopy

J. Wang, T. Yamamoto, J. Bai, S. J. Cox, K. J. Korshavn, M. Monette and A. Ramamoorthy, Chem. Commun., 2018, 54, 2000 DOI: 10.1039/C8CC00167G

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Chemical Communications