Issue 3, 2018

Microfluidic formation of proteinosomes


Herein we describe a novel microfluidic method for the generation of proteinosome micro-droplets, based on bovine serum albumin and glucose oxidase conjugated to PNIPAAm chains. The size of such water-in-oil droplets is regulated via control of the input reagent flow rate, with generated proteinosome populations exhibiting narrower size distributions than those observed when using standard bulk methodologies. Importantly, proteinosomes transferred from an oil to an aqueous-environment remain intact, become fully hydrated and exhibit an increase in average size. Moreover, functional proteinosomes prepared via microfluidics exhibit lower Km values and higher enzymatic activities than proteinosomes produced by bulk methodologies.

Graphical abstract: Microfluidic formation of proteinosomes

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Article information

Article type
03 Nov 2017
06 Dec 2017
First published
06 Dec 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 287-290

Microfluidic formation of proteinosomes

M. Ugrinic, A. Zambrano, S. Berger, S. Mann, T.-Y. D. Tang and A. deMello, Chem. Commun., 2018, 54, 287 DOI: 10.1039/C7CC08466H

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