Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 9, 2018
Previous Article Next Article

Fatty acid profile of stinging nettle leaves: application of modern analytical procedures for sample preparation and analysis

Author affiliations

Abstract

Stinging nettle is a perennial plant with significant biological activity and a long history of application in folk medicine and as food. Its activity is ascribed to its chemical composition. Its leaves contain different classes of compounds including fatty acids. This study deals with the isolation of fatty acids in stinging nettle leaves using a rapid extraction system for solid–liquid extraction (Soxtherm) with petroleum ether as the solvent. The obtained results indicate that the saturated fatty acids are more abundant than unsaturated fatty acids. The most abundant fatty acids are C12 : 0 (20.94%, 348.07 μg g−1), C16 : 0 (18.57%, 309.06 μg g−1), and C18 : 1 (13.16%, 219.44 μg g−1), whereas the fatty acid with the lowest content is C17 : 1 (0.13%, 2.20 μg g−1). Prior to analysis, the method is validated to obtain accurate and precise results. The limit of detection and limit of quantification are 0.012–0.038% and 1.67–7.03%, whereas the precision and accuracy are 83–126% and 1.67–7.03%, respectively. The validation parameters are >0.999, 0.011–0.032%, 0.012–0.038%, 1.67–7.03%, 83–126%, 0.22–5.24%, and 0.38–6.94% for linearity, limit of detection, limit of quantitation, precision, accuracy, inter-day precision, and intra-day precision, respectively, confirming the suitability of this method for fatty acid analysis.

Graphical abstract: Fatty acid profile of stinging nettle leaves: application of modern analytical procedures for sample preparation and analysis

Back to tab navigation

Supplementary files

Article information


Submitted
31 Oct 2017
Accepted
05 Feb 2018
First published
06 Feb 2018

Anal. Methods, 2018,10, 1080-1087
Article type
Paper

Fatty acid profile of stinging nettle leaves: application of modern analytical procedures for sample preparation and analysis

S. Đurović, Z. Zeković, S. Šorgić, S. Popov, M. Vujanović and M. Radojković, Anal. Methods, 2018, 10, 1080
DOI: 10.1039/C7AY02559A

Social activity

Search articles by author

Spotlight

Advertisements