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Field Amplification Enhanced Paper-Based Analytical Device with Robust Chemiluminescence Detection Module

Abstract

Abstract: Sensitive detection method combined with effective on-line concentration may improve the analytical performance of a paper-based analytical device (PAD), and fully demonstrate its merits of low cost and portability in POCT. Here, a sensitive PAD system with chemiluminscence (CL) detection and electrokinetic preconcentration was introduced, and the performance was demonstrated by the detection of hemin. A commercial available low cost and miniaturized optical detection module was used for the CL detection. Firstly, hemin was stacked on a simple paper fluidic channel based on field amplified stacking (FAS), and then CL reagent (luminol-H2O2) was loaded on the stacked band to initiate the CL reaction. The photons were directly detected by the detection module. With optimization of background electrolyte (BGE), voltage and CL reagent, a limit of detection (LOD) of 0.58 nM for hemin was obtained with a linear range of 1~1000 nM (R2=0.995). With FAS, the signal intensity was about 13-fold enhanced. This PAD also exhibited satisfactory selectivity over possible interference components at a concentration of 104 times higher. The applicability was demonstrated by the detection of hemin from iron supplements and human serum samples. With total manual operation, recovery rate of 84.8 ~ 115.6 % was obtained with RSD of less than 14.3 %. With the introduction of the optical detection model, and together with FAS, both of the LOD and dynamic range of this PAD was effectively improved.

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Supplementary files

Publication details

The article was received on 27 Sep 2018, accepted on 02 Nov 2018 and first published on 06 Nov 2018


Article type: Paper
DOI: 10.1039/C8AN01859F
Citation: Analyst, 2018, Accepted Manuscript
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    Field Amplification Enhanced Paper-Based Analytical Device with Robust Chemiluminescence Detection Module

    X. Zhang, J. Liu, Y. Cai, S. Zhao and Z. Wu, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01859F

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