Jump to main content
Jump to site search


Transition metal-Coordinated Graphitic Carbon Nitride Dots as a Sensitive and Facile Fluorescence probe for β-Amyloid Peptide detection

Abstract

Herein, we developed a sensitive graphitic carbon nitride dots (gCNQDs)-based fluorescent strategy for β-amyloid peptide monomer (Aβ) determination down to ng/mL level for the first time. To realize this goal, the nanostructured gCNQDs was firstly coordinated with four transition metal ions (Cu2+, Cu+, Fe3+, Zn2+). Our findings showed that the fluorescence (FL) intensity of gCNQDs was quenched in the presence of these metal ions possibly due to the effective chelating with nitrogen element in gCNQDs and subsequent photoinduced electron transfer (PET) of gCNQDs. The degree of fluorescence quench was found the most intense with addition of Cu2+ and therefore, we selected Cu2+ as the quencher for the following Aβ determination. Through binding to Cu2+, the introduction of Aβ unexpectedly induced further decline of FL intensity. Importantly, on account of different peptide sequences coexisting in the same cerebral system, including Aβ1-11, Aβ1-16, Aβ1-38, Aβ1-40 and Aβ1-42, their affinities to Cu2+ could be reflected by the distinguished declining extent of FL intensity. The possible mechanism of Aβ sensing by the probe was clarified by TEM characterizations. The developed fluorescent biosensor was demonstrated to give a wide linear range from 1 to 700 ng/mL and a low detection limit of 0.18 ng/mL for Aβ1-42. In the end, the proposed fluorescence approach was successfully applied into monitoring of Aβ1-42 variations in the cortex and hippocampus of AD rats.

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Aug 2018, accepted on 01 Nov 2018 and first published on 02 Nov 2018


Article type: Paper
DOI: 10.1039/C8AN01620H
Citation: Analyst, 2018, Accepted Manuscript
  •   Request permissions

    Transition metal-Coordinated Graphitic Carbon Nitride Dots as a Sensitive and Facile Fluorescence probe for β-Amyloid Peptide detection

    Y. Zhang, S. Meng, J. Ding, Q. Peng and Y. Yu, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01620H

Search articles by author

Spotlight

Advertisements