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Issue 24, 2018
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Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480

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Abstract

The development of new imaging tools, molecules and modalities is crucial to understanding biological processes and the localised cellular impact of bioactive compounds. A small molecule photosensitiser, DC473, has been designed to be both highly fluorescent and to exhibit a strong Raman signal in the cell-silent region of the Raman spectrum due to a diphenylacetylene structure. DC473 has been utilised to perform a range of novel tandem fluorescence and Raman (fluoRaman) imaging experiments, enabling a thorough examination of the compound's cellular localisation, exemplified in colorectal cancer cells (SW480). This multifunctional fluoRaman imaging modality revealed the presence of the compound in lipid droplets and only a weak signal in the cytosol, by both Raman and fluorescence imaging. In addition, Raman microscopy detected the compound in a cell compartment we labelled as the nucleolus, whereas fluorescence microscopy did not detect the fluoRaman probe due to solvatochromatic effects in a local polar environment. This last finding was only possible with the use of tandem confocal Raman and fluorescence methods. By following the approach detailed herein, incorporation of strong Raman functional groups into fluorophores can enable a plethora of fluoRaman experiments, shedding further light on potential drug compound's cellular behaviour and biological activity.

Graphical abstract: Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480

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Publication details

The article was received on 31 Jul 2018, accepted on 01 Nov 2018 and first published on 23 Nov 2018


Article type: Paper
DOI: 10.1039/C8AN01461B
Citation: Analyst, 2018,143, 6113-6120
  • Open access: Creative Commons BY license
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    Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480

    J. Gala de Pablo, D. R. Chisholm, A. Steffen, A. K. Nelson, C. Mahler, T. B. Marder, S. A. Peyman, J. M. Girkin, C. A. Ambler, A. Whiting and S. D. Evans, Analyst, 2018, 143, 6113
    DOI: 10.1039/C8AN01461B

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