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A PCR-free technology to detect and quantify microRNAs directly from human plasma

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Abstract

A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a, an important miRNA in erythropoiesis, and compare with the gold standard RT-qPCR.

Graphical abstract: A PCR-free technology to detect and quantify microRNAs directly from human plasma

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Publication details

The article was received on 24 Jul 2018, accepted on 25 Sep 2018 and first published on 27 Sep 2018


Article type: Communication
DOI: 10.1039/C8AN01397G
Citation: Analyst, 2018, Advance Article
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    A PCR-free technology to detect and quantify microRNAs directly from human plasma

    A. Marín-Romero, A. Robles-Remacho, M. Tabraue-Chávez, B. López-Longarela, R. M. Sánchez-Martín, J. J. Guardia-Monteagudo, M. A. Fara, F. J. López-Delgado, S. Pernagallo and J. J. Díaz-Mochón, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN01397G

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