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Cerebrospinal fluid levels of synuclein alpha measured using poly-glutamic acid-modified gold nanoparticle-doped disposable neuro-biosensor system

Abstract

Synuclein (SYN) alpha is a prominent key protein in Parkinson-type dementia. Measurement of the amount of this protein found in cerebrospinal fluid (CSF) using precise techniques may help in the diagnosis and prognosis of the disease. In this study, a gold nanoparticle (AuNP)-polyglutamic acid (PGA)-modified indium tin oxide (ITO)-based disposable neuro-biosensor system was designed for synuclein alpha (SYN-alpha), an important biomarker of Parkinson's disease. Glutamic acid was formed by electropolymerization on the electrode surface. The parameters that can affect the performance of the biosensing prob were optimized. The techniques used in the design of the immobilization steps, the optimization studies, and the evaluation of the analytical performance of the targeted neuro-biosensor are electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and square wave voltammetry (SWV). Charge transfer resistance (Rct) changes were highly linear and sensitive with SYN alpha concentration in the 4-2000 pg/mL range and associated with a limit of detection of 0.135 pg/mL. With the designed disposable neuro-biosensor system, the amount of SYN alpha found in CSF samples was determined by standard addition technique and found to be strikingly sensitive to the target analyte. Morphological and chemical changes on the sensing surface were evaluated by scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). In addition, it was determined that the PGA-modified, AuNP-doped neuro-biosensor system has great reproducibility potency, long storage stability, and regeneration capacity. We suggest that the AuNP-PGA combination platform is ideal for use as a biosensing probe to detect SYN-alpha.

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Supplementary files

Publication details

The article was received on 12 Jul 2018, accepted on 23 Oct 2018 and first published on 29 Oct 2018


Article type: Paper
DOI: 10.1039/C8AN01279B
Citation: Analyst, 2018, Accepted Manuscript
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    Cerebrospinal fluid levels of synuclein alpha measured using poly-glutamic acid-modified gold nanoparticle-doped disposable neuro-biosensor system

    M. N. Sonuc Karaboga and M. K. Sezgintürk, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN01279B

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