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Issue 22, 2018
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Hyphenation of high-temperature liquid chromatography with high-pressure electrospray ionization for subcritical water LC-ESI-MS

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Abstract

High-pressure electrospray ionization (HP-ESI) performed under super-atmospheric pressure allows a stable and efficient electrospray of pure aqueous and/or superheated solutions even under a μL min−1 flow rate regime. In this paper, we report the direct coupling of the HP-ESI source to high-temperature liquid chromatography (HT-LC) operated at ≤30 μL min−1 flow rates. In addition to ESI, the ion source functions as a back-pressure regulator to keep the mobile phase in the liquid phase when the column is heated to >100 °C. Under an ion source pressure of 7 bar, the LC column can be operated up to 160 °C. LC is performed under isocratic elution, and besides the isothermal mode, the temperature of the column can also be programmed to increase the selectivity while keeping the ion source at a constant temperature. For a given solution flow rate, the analytical time can be shortened by increasing the column temperature. HT-LC-ESI-MS using pure water as the mobile phase with a capillary column is also demonstrated.

Graphical abstract: Hyphenation of high-temperature liquid chromatography with high-pressure electrospray ionization for subcritical water LC-ESI-MS

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Publication details

The article was received on 18 Jun 2018, accepted on 20 Sep 2018 and first published on 25 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01113C
Citation: Analyst, 2018,143, 5552-5558

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    Hyphenation of high-temperature liquid chromatography with high-pressure electrospray ionization for subcritical water LC-ESI-MS

    L. C. Chen, T. Naito, S. Ninomiya and K. Hiraoka, Analyst, 2018, 143, 5552
    DOI: 10.1039/C8AN01113C

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