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A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model

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Abstract

Peroxynitrite (ONOO) plays important roles in the regulation of many physiological and pathological processes, and an increase in its levels is related to numerous diseases. Thus, accurate detection of ONOO in physiological conditions is imperative for elucidating its functions. However, studies on high signal-to-noise-ratio (SNR) fluorescence imaging of ONOO in vivo for its detection are currently lacking. Thus, a novel NIR xanthene fluorescence probe (NOF2) for the endogenous detection of ONOO is designed and synthesized. The fluorescence of the NOF2 probe is pre-quenched by the hydroxyl protection group of diphenyl phosphinate. Additionally, the NOF2 probe exhibits good selectivity and sensitivity for ONOO with a low detection limit of 0.40 μM. Importantly, the NOF2 probe displays good performances for the detection of endogenous ONOO not only in living cells but also in a mouse inflammation model. This demonstrates its great potential for applications involving the detection of ONOO both in vitro and in vivo to explore the roles of ONOO in different physiological systems.

Graphical abstract: A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model

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Publication details

The article was received on 16 Jun 2018, accepted on 24 Sep 2018 and first published on 25 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN01107A
Citation: Analyst, 2018, Advance Article
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    A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model

    Y. Wu, A. Shi, Y. Li, H. Zeng, X. Chen, J. Wu and X. Fan, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN01107A

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