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Issue 8, 2018
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Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides

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Abstract

We report here our combination of cryogenic, messenger-tagging, infrared (IR) spectroscopy with ion mobility spectrometry (IMS) and mass spectrometry (MS) as a way to identify and analyze a set of human milk oligosaccharides (HMOs) ranging from trisaccharides to hexasaccharides. The added dimension of IR spectroscopy provides a diagnostic fingerprint in the OH and NH stretching region, which is crucial to identify these oligosaccharides, which are difficult to distinguish by IMS alone. These results extend our previous work in demonstrating the generality of this combined approach for distinguishing subtly different structural and regioisomers of glycans of biologically relevant size.

Graphical abstract: Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides

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Article information


Submitted
05 Feb 2018
Accepted
06 Mar 2018
First published
07 Mar 2018

Analyst, 2018,143, 1846-1852
Article type
Paper

Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides

N. Khanal, C. Masellis, M. Z. Kamrath, D. E. Clemmer and T. R. Rizzo, Analyst, 2018, 143, 1846
DOI: 10.1039/C8AN00230D

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