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Issue 5, 2017
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Highly specific and rapid glycan based amperometric detection of influenza viruses

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Abstract

Rapid and precise detection of influenza viruses in a point of care setting is critical for applying appropriate countermeasures. Current methods such as nucleic acid or antibody based techniques are expensive or suffer from low sensitivity, respectively. We have developed an assay that uses glucose test strips and a handheld potentiostat to detect the influenza virus with high specificity. Influenza surface glycoprotein neuraminidase (NA), but not bacterial NA, cleaved galactose bearing substrates, 4,7di-OMe N-acetylneuraminic acid attached to the 3 or 6 position of galactose, to release galactose. In contrast, viral and bacterial NA cleaved the natural substrate, N-acetylneuraminic acid attached to the 3 or 6 position of galactose. The released galactose was detected amperometrically using a handheld potentiostat and dehydrogenase bearing glucose test strips. The specificity for influenza was confirmed using influenza strains and different respiratory pathogens that include Streptococcus pneumoniae and Haemophilus influenzae; bacteria do not cleave these molecules. The assay was also used to detect co-infections caused by influenza and bacterial NA. Viral drug susceptibility and testing with human clinical samples was successful in 15 minutes, indicating that this assay could be used to rapidly detect influenza viruses at primary care or resource poor settings using ubiquitous glucose meters.

Graphical abstract: Highly specific and rapid glycan based amperometric detection of influenza viruses

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Supplementary files

Article information


Submitted
18 Aug 2016
Accepted
13 Feb 2017
First published
14 Feb 2017

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2017,8, 3628-3634
Article type
Edge Article

Highly specific and rapid glycan based amperometric detection of influenza viruses

X. Cui, A. Das, A. N. Dhawane, J. Sweeney, X. Zhang, V. Chivukula and S. S. Iyer, Chem. Sci., 2017, 8, 3628
DOI: 10.1039/C6SC03720H

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