Selective growth inhibition of cancer cells with doxorubicin-loaded CB-modified iron-oxide nanoparticles
Cucurbituril-modified iron-oxide nanoparticles (CBNPs) were loaded with doxorubicin hydrochloride (Dox) and tested as a drug delivery system. Dox was found to interact with the carbonyl-rich rims of the CB macrocycles adsorbed on the surface of the nanoparticles. The Dox-loaded nanoparticles (Dox@CBNPs) were stable at room temperature and physiological pH and released their Dox cargo under acidic conditions, in the presence of glutathione, or with heating. Dox@CBNPs reduced the viability of HeLa and three other cancer-derived cell lines in vitro at lower IC50 than free Dox. They were also nontoxic to C. elegans. The sensitivity of HeLa cells to Dox@CBNPs was enhanced when the temperature was elevated by application of an alternating magnetic field. Thus, Dox@CBNPs show promise as agents for the intracellular delivery of Dox to cancer cells, for the selective and controlled release of the drug, and, more generally, as a possible means of combining chemotherapeutic and hyperthermic treatment modalities.