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Issue 65, 2017, Issue in Progress
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Endogenous omega-3 long-chain fatty acid biosynthesis from alpha-linolenic acid is affected by substrate levels, gene expression, and product inhibition

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Abstract

Previous studies have suggested that dietary alpha-linolenic acid (ALA) increases the levels of omega-3 long-chain polyunsaturated fatty acids (ω-3 LC-PUFAs) in vivo, but the conversion procedure and the genes involved remain poorly understood. In the present work, we designed diets containing various concentrations of ALA and eicosapentaenoic acid (EPA) to feed to mice. Dietary ALA increased the ALA levels in the body in a linear manner and also increased the ω-3 LC-PUFA concentration, but higher ALA intake (above 5%) had no additional effect on ω-3 LC-PUFA levels in vivo. Dietary ALA at a moderate level increased the expression of genes such as Fads1, Fads2, and Elovl5, but higher levels of dietary ALA (above 5%) inhibited their expression in the liver. Further studies demonstrated that the converted EPA could also inhibit the expression of these genes in a concentration-dependent manner, which illustrated that Fads1, Fads2, and Elovl5 were the key genes involved in the conversion of ALA to ω-3 LC-PUFAs. Endogenous ω-3 LC-PUFA biosynthesis from ALA was affected by substrate level, gene expression, and product inhibition.

Graphical abstract: Endogenous omega-3 long-chain fatty acid biosynthesis from alpha-linolenic acid is affected by substrate levels, gene expression, and product inhibition

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Publication details

The article was received on 16 Jun 2017, accepted on 29 Jul 2017 and first published on 22 Aug 2017


Article type: Paper
DOI: 10.1039/C7RA06728C
RSC Adv., 2017,7, 40946-40951
  • Open access: Creative Commons BY license
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    Endogenous omega-3 long-chain fatty acid biosynthesis from alpha-linolenic acid is affected by substrate levels, gene expression, and product inhibition

    S. Wang, Y. Pan, J. Li, H. Chen, H. Zhang, W. Chen, Z. Gu and Y. Q. Chen, RSC Adv., 2017, 7, 40946
    DOI: 10.1039/C7RA06728C

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