Issue 63, 2017

Comparative studies on the interaction of nitrofuran antibiotics with bovine serum albumin

Abstract

The interactions of typical nitrofuran antibiotics, nitrofurazone (NFZ) and nitrofurantoin (NFT), with bovine serum albumin (BSA) have been studied using fluorescence and FT-IR spectroscopy, circular dichroism (CD), atomic force microscopy (AFM) and molecular docking investigation. The results revealed that the fluorescence of BSA was quenched by nitrofurans with a static quenching mechanism. The conformation of BSA was changed and unfolded with the addition of nitrofurans because the alpha-helix and beta-sheet were varied to beta-turn and random structure. This study also indicated that the impact on the conformational changes of BSA caused by NFZ was more conspicuous than that of NFT. The molecular simulation studies were carried out to clearly describe the nature of the interaction between nitrofurans and BSA.

Graphical abstract: Comparative studies on the interaction of nitrofuran antibiotics with bovine serum albumin

Supplementary files

Article information

Article type
Paper
Submitted
17 May 2017
Accepted
13 Jul 2017
First published
15 Aug 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 39833-39841

Comparative studies on the interaction of nitrofuran antibiotics with bovine serum albumin

Q. Zhang and Y. Ni, RSC Adv., 2017, 7, 39833 DOI: 10.1039/C7RA05570F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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