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Issue 56, 2017, Issue in Progress
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Slipdisc: a versatile sample preparation platform for point of care diagnostics

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Abstract

We report a microfluidic sample preparation platform called “Slipdisc” based on slipchip technology. Slipdisc is a rotational slipchip that uses a unique hand-wound clockwork mechanism for precise movement of specially fabricated polycarbonate discs. In operation, the microchannels and microchambers carved on the closely aligned microfluidic discs convert from continuous filled paths to defined compartments using the slip movement. The clockwork mechanism introduced here is characterised by a food dye experiment and a conventional HRP TMB reaction before measuring lactate dehydrogenase (LDH) enzyme levels, which is a crucial biomarker for neonatal diagnostics. The colorimetry based detection of LDH was performed with an unmodified camera and an image analysis procedure based on normalising images and observing changes in red channel intensity. The analysis showed a close to unity coefficient of determination (R2 = 0.96) in detecting the LDH concentration when compared with a standard Chemical Analyser, demonstrating the excellent performance of the slipdisc platform with colorimetric detection. The versatile point of care sample preparation platform should ideally be suited for a multitude of applications at resource-limited settings.

Graphical abstract: Slipdisc: a versatile sample preparation platform for point of care diagnostics

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Publication details

The article was received on 09 May 2017, accepted on 26 Jun 2017 and first published on 12 Jul 2017


Article type: Paper
DOI: 10.1039/C7RA05209J
Citation: RSC Adv., 2017,7, 35048-35054
  • Open access: Creative Commons BY license
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    Slipdisc: a versatile sample preparation platform for point of care diagnostics

    I. Banerjee, T. Salih, H. Ramachandraiah, J. Erlandsson, T. Pettersson, A. C. Araújo, M. Karlsson and A. Russom, RSC Adv., 2017, 7, 35048
    DOI: 10.1039/C7RA05209J

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