Jump to main content
Jump to site search

Issue 28, 2017, Issue in Progress
Previous Article Next Article

Integration of heterologous 4-hydroxybenzoic acid transport proteins in Rhodobacter sphaeroides for enhancement of coenzyme Q10 production

Author affiliations

Abstract

The highly redox-active compound, Coenzyme Q10 (CoQ10), has attracted increasing interest due to its clinical benefits and important applications in the pharmaceutical, food and health industries. Although the metabolic pathways involved in CoQ10 biosynthesis are known, very few reports are available concerning the development of engineering strategies to enhance CoQ10 production in the natural producer Rhodobacter sphaeroides. In this work, three membrane transport proteins, AcPcaK, KpPcaK, and CgPcaK from different organisms (Acinetobacter calcoaceticus, Klebsiella pneumoniae and Corynebacterium glutamicum, respectively) were individually heterologously expressed in R. sphaeroides GY-2 to enhance the uptake of extracellular 4-hydroxybenzoic acid (4HBA), an important intermediate of CoQ10 synthesis. Each of the PcaK open reading frames (ORFs) was inserted behind an RFP gene to weaken their expression in order to avoid inhibition of cell growth. The transport efficiency and consumption of 4HBA, as well as CoQ10 productivity of the resulting recombinant R. sphaeroides GY-2 strains has been studied. The CoQ10 productivity of RS-CgPcaK and RS-KpPcaK was effectively improved by the addition of external 4HBA, reaching maximum values of 17.45 and 18.06 mg gāˆ’1 DCW (18.05% and 20.82% higher than R. sphaeroides GY-2), respectively. The strategy used in this study proved effective for enhancing the biotechnological production of CoQ10 and thus holds promise for further improvements, including industrial applications.

Graphical abstract: Integration of heterologous 4-hydroxybenzoic acid transport proteins in Rhodobacter sphaeroides for enhancement of coenzyme Q10 production

Back to tab navigation

Article information


Submitted
25 Feb 2017
Accepted
13 Mar 2017
First published
20 Mar 2017

This article is Open Access

RSC Adv., 2017,7, 17346-17352
Article type
Paper

Integration of heterologous 4-hydroxybenzoic acid transport proteins in Rhodobacter sphaeroides for enhancement of coenzyme Q10 production

F. Qi, L. Zou, X. Jiang, S. Cai, M. Zhang, X. Zhao and J. Huang, RSC Adv., 2017, 7, 17346
DOI: 10.1039/C7RA02346D

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements