Issue 39, 2017, Issue in Progress

Label-free detection of anti-estrogen receptor alpha and its binding with estrogen receptor peptide alpha by terahertz spectroscopy

Abstract

Terahertz (THz) spectroscopic techniques were employed to study the hydration shell formation around anti-estrogen receptor alpha (AER-α), an important biomarker in breast cancer diagnosis, and to detect the binding reaction between AER-α and estrogen receptor peptide alpha (ERP-α), in aqueous solutions. A remarkable nonlinear relation between THz absorption and AER-α concentration was demonstrated, shedding some light on the formation process of the hydration shell around AER-α. THz spectroscopic properties of the AER-α were also shown to be significantly affected by the binding with the ERP-α, to the extent that THz transmission and attenuation could be used to investigate the dielectric properties of antibody–antigen binding reactions.

Graphical abstract: Label-free detection of anti-estrogen receptor alpha and its binding with estrogen receptor peptide alpha by terahertz spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
27 Dec 2016
Accepted
20 Apr 2017
First published
04 May 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 24338-24344

Label-free detection of anti-estrogen receptor alpha and its binding with estrogen receptor peptide alpha by terahertz spectroscopy

M. Li, T. Chang, D. Wei, M. Tang, S. Yan, C. Du and H. Cui, RSC Adv., 2017, 7, 24338 DOI: 10.1039/C6RA28754A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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