Issue 32, 2017, Issue in Progress

Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

Abstract

For the first time, we have engineered streptavidin labeled fluorescent silver nanoparticles for their application in immunosensing of biomolecules which will significantly increase sensitivity without compromising the specificity. A computational perspective for understanding the efficiency of the conjugation process is provided as a proof of concept. The fluorescence based sandwich immunoassay was demonstrated to detect HIV-1 p24 antigen in clinical specimens with improved sensitivity and specificity. The detection range of the fluorescent silver nanoparticle-based immunoassay (FSNIA) was found to be between 10 and 1000 pg mL−1 in a linear dose dependent manner. False positives were not observed with plasma samples from healthy adults (HIV−ve), hepatitis B (HBV+ve) and hepatitis C (HCV+ve). Plasma samples that were HIV−ve showed no interference with detection of HIV-1 p24 antigen. This technology can be used in resource limited settings and easily adopted for the detection of other pathogen antigens.

Graphical abstract: Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

Supplementary files

Article information

Article type
Paper
Submitted
27 Dec 2016
Accepted
14 Mar 2017
First published
05 Apr 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 19863-19877

Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

A. D. Kurdekar, L. A. A. Chunduri, S. M. Chelli, M. K. Haleyurgirisetty, E. P. Bulagonda, J. Zheng, I. K. Hewlett and V. Kamisetti, RSC Adv., 2017, 7, 19863 DOI: 10.1039/C6RA28737A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements