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Issue 15, 2017
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Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

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Abstract

Established laser-based ionization experiments require the surface of a sample to be as flat as possible to guarantee optimal laser focus. A laser ablation electrospray ionization (LAESI) source was custom-built to accommodate the topography of non-flat sample surfaces. Employing a confocal distance sensor, a height profile of the surface in question was recorded prior to the actual ionization experiment. The robustness of the system was evaluated by the metabolic profiling of radish (Raphanus sativus) leaves, chosen due to their pronounced surface features and known content of specialized metabolites. After the ionization experiments, light microscopy imaging was performed to evaluate ablation crater size and position. Reproducible ablation mark diameters of 69 ± 7 μm in average have been achieved. Mass spectrometric imaging capability has been proven on R. sativus leaf samples as well.

Graphical abstract: Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

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Publication details

The article was received on 15 Nov 2016, accepted on 12 Jan 2017 and first published on 27 Jan 2017


Article type: Paper
DOI: 10.1039/C6RA26854D
Citation: RSC Adv., 2017,7, 9045-9050
  • Open access: Creative Commons BY license
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    Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

    B. Bartels, P. Kulkarni, N. Danz, S. Böcker, H. P. Saluz and A. Svatoš, RSC Adv., 2017, 7, 9045
    DOI: 10.1039/C6RA26854D

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