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Issue 2, 2017
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A biocompatible macromolecular two-photon initiator based on hyaluronan

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Abstract

The possibility of the direct encapsulation of living cells via two-photon induced photopolymerization enables the microfabrication of hydrogel scaffolds with high initial cell loadings and intimate matrix–cell contact. While highly efficient water-soluble two-photon initiators based on benzylidene ketone dyes have been developed, they exhibit considerable cyto- and phototoxicity. To address the problem of photoinitiator migration from the extracellular matrix into the cytosol, a two-photon initiator bound to a polymeric hyaluronan backbone (HAPI) was synthesized in this work. HAPI exhibited a distinct improvement of cytocompatibility compared to a reference two-photon initiator. Basic photophysical investigations were performed to characterize the absorption and fluorescence behavior of HAPI. Laser scanning microscopy was used to visualize and confirm the hindered transmembrane migration behavior of HAPI. The performance of HAPI was tested in two-photon polymerization at exceedingly high printing speeds of 100 mm s−1 producing gelatin-based complex 3D hydrogel scaffolds with a water content of 85%. The photodamage of the structuring process was low and viable MC3T3 cells embedded in the gel were monitored for several days after structuring.

Graphical abstract: A biocompatible macromolecular two-photon initiator based on hyaluronan

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Publication details

The article was received on 12 Oct 2016, accepted on 21 Nov 2016 and first published on 29 Nov 2016


Article type: Paper
DOI: 10.1039/C6PY01787H
Polym. Chem., 2017,8, 451-460
  • Open access: Creative Commons BY license
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    A biocompatible macromolecular two-photon initiator based on hyaluronan

    M. Tromayer, P. Gruber, M. Markovic, A. Rosspeintner, E. Vauthey, H. Redl, A. Ovsianikov and R. Liska, Polym. Chem., 2017, 8, 451
    DOI: 10.1039/C6PY01787H

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