Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 40, 2017
Previous Article Next Article

Multifunctional gold nanoparticle layers for controllable capture and release of proteins

Author affiliations

Abstract

Protein modified functional surfaces have been applied extensively in the field of biomaterials and medicine. Regulation of the amount and activity of proteins on the material surface is always a challenge and a key research issue. A multifunctional micro/nano-composite based surface system for efficient controllable capture and release of proteins is proposed and studied in the present paper. This novel system contains (1) gold nanoparticles (AuNPs) co-modified with an enzyme and poly(methacrylic acid) (PMAA), e.g., AuNP-pyrophosphatase (PPase)-PMAA, as nanostructured protein carriers; (2) gold nanoparticle layers (GNPLs) modified with poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA), i.e., GNPL-PDMAEMA, as a micro/nano-structured support platform for surface bioactivity regulation. The capture–release of proteins and the regulation of surface bioactivity in this composite surface system were investigated under different conditions. The results showed that the proposed system is capable of protein capture and release with simple adjustment of the pH value from neutral pH to basic pH. When the pH of the system is stabilized at 7.0, the GNPL-PDMAEMA surface could adsorb plenty of AuNP-PPase-PMAA conjugates and maximum surface bioactivity occurred, but when the pH of the system is adjusted to 10.0, the GNPL-PDMAEMA surface could liberate almost all the AuNP-PPase-PMAA conjugates and thus surface bioactivity disappeared. Meanwhile, by cyclical variations between pH 7.0 and pH 10.0, this surface protein capture/release system could realize recycling and reuse of one certain protein multiple times, a series of proteins acting sequentially in accordance with pre-designed procedures, and a functional combination of multiple proteins. This recyclable multifunctional surface with the capability of protein capture/release has great potential in many applications, such as biomonitoring and biomolecule immobilization.

Graphical abstract: Multifunctional gold nanoparticle layers for controllable capture and release of proteins

Back to tab navigation

Supplementary files

Article information


Submitted
19 Jul 2017
Accepted
10 Sep 2017
First published
11 Sep 2017

Nanoscale, 2017,9, 15407-15415
Article type
Paper

Multifunctional gold nanoparticle layers for controllable capture and release of proteins

Z. Li, F. Liu, Y. Yuan, J. Wu, H. Wang, L. Yuan and H. Chen, Nanoscale, 2017, 9, 15407
DOI: 10.1039/C7NR05276F

Social activity

Search articles by author

Spotlight

Advertisements