Jump to main content
Jump to site search

Issue 4, 2017
Previous Article Next Article

Design of SERS nanotags for multiplexed lateral flow immunoassays

Author affiliations

Abstract

Surface enhanced Raman spectroscopy (SERS) has been attractive for enhancing the sensitivity of lateral flow immunoassays (LFA). A format that has enabled specific detection of biomarkers is to use Raman reporter molecules linked to gold nanoparticles (NPs), which are conjugated to laboratory made antibodies specific for the target of interest. Many factors such as the NP and Ab properties and the method of signal readout impact the sensitivity of a SERS based immunoassay. To understand how to optimize assay sensitivity, we studied SERS readouts of multiplexed sandwich immunoassays for the zika and dengue non-structural protein 1 (NS1) biomarkers as a test case. We investigated the effect of NP shape on the SERS enhancement of the reporter molecules 1,2-bis(4-pyridyl)ethylene (BPE) and 4-mercaptobenzoic acid (MBA). We also performed SERS imaging of test lines to map the spatial distribution of signal in test lines on the nitrocellulose. Finally, we used a modified least squares analysis to differentiate reporter contributions.

Graphical abstract: Design of SERS nanotags for multiplexed lateral flow immunoassays

Back to tab navigation

Supplementary files

Article information


Submitted
07 Jul 2017
Accepted
21 Sep 2017
First published
27 Sep 2017

Mol. Syst. Des. Eng., 2017,2, 401-409
Article type
Paper

Design of SERS nanotags for multiplexed lateral flow immunoassays

M. Sánchez-Purrà, B. Roig-Solvas, A. Versiani, C. Rodriguez-Quijada, H. de Puig, I. Bosch, L. Gehrke and K. Hamad-Schifferli, Mol. Syst. Des. Eng., 2017, 2, 401
DOI: 10.1039/C7ME00052A

Social activity

Search articles by author

Spotlight

Advertisements