Issue 76, 2017
From the journal:

Chemical Communications

Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching†

Fei Ma,‡a   Wen-jing Liu,‡a   Qianyi Zhang‡b  and  Chun-yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86 0531-82615258
Tel: +86 0531-86186033

b Nantou High School Shenzhen, Shenzhen, China

Abstract

Herein, we develop a simple fluorescence method for the sensitive detection of microRNAs based on duplex specific nuclease-assisted target recycling and pyrene excimer switching. This method is extremely sensitive with a detection limit of 0.58 fM, and it can be applied to absolutely detect endogenous microRNAs in cancer cells, holding great potential for clinical diagnosis and biomedical research studies.

Graphical abstract: Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching

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Article information

DOI
https://doi.org/10.1039/C7CC06290G
Article type
Communication
Submitted
11 Aug 2017
Accepted
05 Sep 2017
First published
05 Sep 2017

Chem. Commun., 2017,53, 10596-10599

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Sensitive detection of microRNAs by duplex specific nuclease-assisted target recycling and pyrene excimer switching

F. Ma, W. Liu, Q. Zhang and C. Zhang, Chem. Commun., 2017, 53, 10596 DOI: 10.1039/C7CC06290G

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