One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

Yuanyuan Sun; Hui Tian; Chenghui Liu; Yueying Sun; Zhengping Li

doi:10.1039/C7CC06140D

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One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)†

Yuanyuan Sun,^a Hui Tian,^a Chenghui Liu,

*^a Yueying Sun^a and Zhengping Li

*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, Shaanxi Province, P. R. China
E-mail: liuch@snnu.edu.cn, lzpbd@snnu.edu.cn
Fax: +86 29 81530859
Tel: +86 29 81530859

Abstract

A novel one-step microRNA assay is developed based on a target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism, which enables the accurate detection of as low as 100 aM (1 zmol) microRNA with simple one-step operation by using only one-type of DNA polymerase.

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Article information

DOI: https://doi.org/10.1039/C7CC06140D
Article type: Communication
Submitted: 06 Aug 2017
Accepted: 18 Sep 2017
First published: 18 Sep 2017

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Chem. Commun., 2017,53, 11040-11043

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One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

Y. Sun, H. Tian, C. Liu, Y. Sun and Z. Li, Chem. Commun., 2017, 53, 11040 DOI: 10.1039/C7CC06140D

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Chemical Communications

One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)†

Abstract

Supplementary files

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One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

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